Fig. 5. AcrB is required for AcrA suppressor-induced opening of the mutant TolC aperture.
A. Vancomycin sensitivity was determined, as described in Fig. 4 legend, from 18 different genetic backgrounds shown in the graph.
B. An active AcrB protein is not required for the AcrAS83G-mediated vancomycin sensitivity. Average inhibition zone diameters were plotted from three independent experiments, with zones varying no greater than 10%.