Fig. 7.

Effects of different AcrA suppressors on efflux function and TolC aperture were assessed by measuring novobiocin and vancomycin sensitivities, respectively, in a background expressing TolC turn 1 mutant (TolC_147AGSG150). Inhibition zones were also measured in AcrA⁺ control strains expressing no TolC (TolC⁻), wild type TolC (TolC-WT) and TolC turn 1 mutant (TolC_147AGSG150). Zones of inhibition around disks soaked with novobiocin (30 µg) and vancomycin (75 µg) were measured after incubating plates for 16 h at 37°C. Average inhibition zone diameters from three independent experiments were plotted, with zones varying no greater than 10%.