Table 2.
Sensitivity of wild type, mutant TolC and mutant TolC containing AcrA suppressors to various inhibitors.
| Sensitivity to inhibitorsb | ||||
|---|---|---|---|---|
| TolC and AcrA proteinsa |
Novobiocin | Erythromycin | CCCP | HlyA |
| TolCWT AcrAWT | 15.80 | 61.60 | 11.20 | + |
| TolCQ AcrAWT | 1.00 | 1.82 | 0.97 | − |
| TolCQ AcrAS83G | 2.00 | 7.15 | 1.05 | − |
| TolCQ AcrAT 111P | 3.60 | 24.60 | 2.50 | − |
| TolCQ AcrAA135T | 3.80 | 13.50 | 1.20 | − |
| TolCQ AcrAT153P | 7.70 | 13.70 | 2.70 | − |
| TolCQ AcrAL252R | 14.50 | 31.10 | 4.20 | − |
a
TolCQ denotes TolC turn 1 147AGSG150 quadruple mutant.
b
Numbers for novobiocin, erythromycin and CCCP represent minimum inhibitory concentrations. Plus and minus signs indicate presence (+) or absence (−) of hemolytic zones on blood agar medium. For hemolysin sensitivity tests, strains shown in the table were transformed with a plasmid expressing the entire hemolysin operon.