Figure 17.9.6.
Simulations of FRET for multiprotein complexes undergoing a conformational change. (A) Scale diagram of a complex of 90- versus 100-Å diameter. The modeled complex is of three antigens around a circle of the indicated diameter. (B) Clusters of three antigens in circles of diameters ranging from 9 to 11-nm were randomly distributed in 0.25 μm2 areas, and the FRET between the bound antibodies calculated by Monte Carlo simulations as in Figure 17.9.5. Each set of 20 measurements was statistically significant from an adjacent set.