Figure 7.
Separation of peptide mixture using a packed Acclaim PepMap™ C18 Nanocolumn. Conditions: column 15 cm × 75 μm I.D., concentration of each peptide in solution 0.1 mg/mL, injection volume 100 nL, flow rate 0.5 μL/min, mobile phase A, aqueous 0.1% (v/v) formic acid, B, 0.1% (v/v) formic acid in acetonitrile, gradient 0-60 % B in A in 8 min, UV detection at 214 nm. Peaks: [Cys17]-β-aamyloid (1-17) (1); β-amyloid (1-17) (2), His-Ala-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Cys (3), LH-RH (4), His-Cys-Lys-Phe-Trp-Trp (5), Tyr-Gly-Gly-Phe-Leu (6).