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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Gene Ther. 2009 Nov 5;17(2):281–287. doi: 10.1038/gt.2009.132

Figure 2.

Figure 2

Viral gene expression of vaccinia virus in human pancreatic cancer after viral infection, and Hif-1α stabilisation and nuclear translocation in normoxia or hypoxia. A, Hypoxia does not affect viral gene expression of vaccinia virus. Cells were maintained in normoxia or hypoxia prior to and after viral infection. Cells were infected with VVLister at an MOI=1. Vaccinia virus protein was measured using an anti-vaccinia polyclonal antibody. Human PCNA was used as a loading control; B, Hif-1α stabilisation and nuclear translocation was demonstrated on nuclear lysates from MiaPaca2 cells in normoxia (20% pO2) or hypoxia (1% pO2) over a time course. β-actin was used as a loading control.