Figure 4.

Effect of hypoxia on cytotoxicity of Vaccinia Virus Lister Strain. Pancreatic cancer cell lines were pre-treated with hypoxia or normoxia for at least 16 h, then infected with serial dilutions of VVLister and maintained under the same oxygen tension. The infected cells were assayed with MTS reagents at days 6 post infection in 37°C with 5% CO₂ for 2 h. Viable cells were determined as a percentage of the non-infected controls and non linear regression analysis was used to draw dose response curves. Each assay contained six replicates and results are presented as mean +/− standard deviation of four independent experiments.