Table 1.
Optical density measurements in multiple brain regions in virgin and pregnant mice
| Hippocampal formation | Virgin | Pregnant | Two-way ANOVA |
||
|---|---|---|---|---|---|
| Gestational stage | Animal | Interaction | |||
| Dentate gyrus | 69.8 ± 2.0 | 40.6 ± 1.8 | |||
| n (sections) | 138 | 138 | |||
| n (mice) | 3 | 3 | F(330.3, 1, 270), 9.30 × 10−49 | F(7.337, 2, 270), 7.89 × 10−4 | F(4.566, 2, 270), 1.12 × 10−2 |
| CA1 | 25.2 ± 2.2 | 16.3 ± 2.0 | |||
| n (sections) | 64 | 61 | |||
| n (mice) | 3 | 3 | F(25.59, 1, 119), 1.55 × 10−6 | F(10.55, 2, 119), 6.05 × 10−5 | F(3.234, 2, 119), 4.29 × 10−2 |
| Cortex | 65.1 ± 2.1 | 69.1 ± 2.0 | |||
| n (sections) | 105 | 105 | |||
| n (mice) | 3 | 3 | F(2.614, 1, 204), 1.07 × 10−1 | F(16.73, 2, 204), 1.87 × 10−7 | F(6.865, 2, 204), 1.30 × 10−3 |
| Striatum | 168.7 ± 3.9 | 135.1 ± 3.7 | |||
| n (sections) | 74 | 84 | |||
| n (mice) | 3 | 3 | F(115.6, 1, 152), 2.09 × 10−20 | F(45.71, 2, 152), 2.86 × 10−16 | F(2.915, 2, 152), 5.72 × 10−2 |
| Thalamus | |||||
| VL | 162.2 ± 5.6 | 145.0 ± 5.0 | F(14.41, 1, 73), 3.01 × 10−4 | F(5.346, 2, 73), 6.81 × 10−3 | F(0.3652, 2, 73), 6.95 × 10−1 |
| VA | 126.3 ± 7.2 | 108.2 ± 6.1 | F(10.19, 1, 73), 2.08 × 10−3 | F(1.981, 2, 73), 1.45 × 10−1 | F(0.1531, 2, 73), 8.58 × 10−1 |
| VPL | 159.7 ± 4.6 | 129.1 ± 5.7 | F(35.48, 1, 73), 8.34 × 10−8 | F(2.301, 2, 73), 1.07 × 10−1 | F(1.887, 2, 73), 1.59 × 10−1 |
| n (sections) | 37 | 42 | |||
| n (mice) | 3 | 3 | |||
The average optical density was determined for each individual animal, and the average optical density from each animal was used for statistical analysis. Statistical significance was determined using a two-way ANOVA for gestational stage and individual variability. The F values are given as F(sum of squares, degrees of freedom, mean square). Bold F values denote significance at p < 0.05.