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. Author manuscript; available in PMC: 2010 May 24.
Published in final edited form as: Photochem Photobiol. 2007 Nov–Dec;83(6):1405–1414. doi: 10.1111/j.1751-1097.2007.00180.x

Table 3.

The fluorescence decay parameters for the complexes resulting from the mixture of TL mutants (5 μm) and ANS (15 μm).

α1 α2 α3 τ1 (ns)* τ2 (ns) τ3 (ns) λmax (nm) f1 f2 f3 χ2
E34W 0.72 0.11 0.17 0.25 3.06 15.14 465 0.06 0.11 0.83 1.1
M39W 0.62 0.15 0.23 0.25 4.37 15.77 466 0.03 0.15 0.82 1.1
G47W 0.55 0.14 0.31 0.25 3.72 15.62 468 0.02 0.10 0.88 1.1
M55W 0.71 0.13 0.16 0.25 3.50 15.24 465 0.06 0.14 0.80 1.1
A66W 0.78 0.10 0.12 0.25 3.62 15.95 472 0.07 0.14 0.79 1.1
H84W 0.75 0.14 0.11 0.25 3.77 15.72 472 0.07 0.21 0.72 1.0
I88W 0.71 0.14 0.15 0.25 3.88 16.51 467 0.05 0.17 0.78 1.1
F99W 0.54 0.15 0.31 0.25 4.06 15.50 467 0.03 0.11 0.86 1.0
C101W 0.47 0.14 0.39 0.25 3.70 14.62 469 0.02 0.08 0.90 1.0
G103W 0.64 0.18 0.18 0.25 2.72 14.01 475 0.05 0.15 0.80 1.1
E104W 0.64 0.12 0.24 0.25 3.77 15.70 466 0.03 0.11 0.86 1.0
L105W 0.46 0.19 0.35 0.25 4.12 17.42 469 0.02 0.11 0.87 1.2

The measurements were conducted at emission λmax for each complex.

*

was fixed in fluorescence decay analysis.