Table 1.

Phospholipase-induced injury to gastrointestinal cells.

	AGS cells		IEC-6 cells
Phospholipase	LDH	MTT	LDH	MTT
Bee venom PLA2
0.1 U/ml	100 ± 3	106 ± 2	104 ± 3	111 ± 2
1 U/ml	107 ± 3	113 ± 2	97 ± 8	115 ± 3
10 U/ml	100 ± 1	106 ± 2	100 ± 2	111 ± 1
Snake venom PLA2
0.1 U/ml	98 ± 1	97 ± 3	100 ± 3	109 ± 5
1 U/ml	102 ± 2	101 ± 2	95 ± 10	121 ± 7
10 U/ml	102 ± 5	111 ± 2	140 ± 26	94 ± 7
PLC
0.1 U/ml	149 ± 5*	79 ± 3*	292 ± 20*	69 ± 1
1 U/ml	423 ± 5*	12 ± 0.1*	464 ± 2*	17 ± 1*
10 U/ml	446 ± 44*	12 ± 0.1*	473 ± 7*	15 ± 0.1*

AGS or IEC-6 cells were incubated for 24 h with 0.1, 1 or 10 U/ml of the indicated phospholipases. LDH and MTT were measured as described in the Methods. Values are expressed as the percent of control ± standard error of the mean for four separate studies.

^*= p<0.05 versus Control