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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Thorac Cardiovasc Surg. 2009 Oct 9;139(6):1587–1593. doi: 10.1016/j.jtcvs.2009.08.016

Figure 1.

Figure 1

OPN mRNA expression in human lung cancer and immortalized bronchial epithelial cells. Primers amplify each of the three isoforms which are then separated by electrophoresis on 2% agarose gel. OPNa produces the 305 bp dominant band seen at the top. OPNb amplifies a 263 bp fragment seen below the dominant band in A549, H157, Calu-3, H460, and H1299. OPNc amplifies a 224 bp fragment that migrates below the OPNb and is not seen in these cell lines. Amplification of pcmv2 plasmid specific to each isoform serves as positive controls and amplification of PPIA serve as control for equal loading. OPN expression correlates with OPN secretion as detected by ELISA after 72 hours in serum-free media. (BrEpC: immortalized bronchial epithelial cells, adeno: adenocarcinoma, SCLC: small cell NSCLC, NSCLC: non-small cell NSCLC, PPIA:peptidylprolyl isomerase A).