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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Thorac Cardiovasc Surg. 2009 Oct 9;139(6):1587–1593. doi: 10.1016/j.jtcvs.2009.08.016

Figure 5.

Figure 5

Gene array analysis demonstrated OPNa overexpression was associated with a two-fold increase in relative expression of VEGF while OPNc overexpression was associated with a 40% decrease in VEGF expression compared to controls. RT-PCR validation of gene array with regard to divergent VEGF expression with overexpression of the OPN isoforms is demonstrated here. Overexpression of OPNa in all four cell lines resulted in elevated VEGF mRNA expression, while OPNc overexpression decreased VEGF expression in all cell lines compared to wild type cells. Elevated VEGF mRNA expression was appreciated in control cell lines with high native OPN (A549 and H460) compared to cell lines with low native OPN expression (H153 and H358).