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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Bone. 2010 Mar 3;46(6):1533–1545. doi: 10.1016/j.bone.2010.02.024

Fig. 1.

Fig. 1

3.6Col1a1 targets odontoblasts at day 1 pp (P1) (A-fluorescent, B-light field microscopy) and in situ hybridization of BMP4 mRNA in representative sections of 12 day old mouse maxillary first molar odontoblasts (C, D) and osteoblasts (F, G) in BMP4 control (C, F) and BMP4 cKO mice (D, G). 3.6Col1a1-cre targeting at functional odontoblasts is confirmed by the cross of 3.6Col1a1-cre line with ZEG-Reporter. The activated GFP expression observed in odontoblasts of 1 day old mice (A-red arrows) shows that Cre is already activated in odontoblasts after Cre-LoxP recombination at birth. Note that GFP expression is not activated in ameloblasts (A-yellow arrows). The hybridization signal (blue color) in odontoblasts and osteoblasts is reduced 94% in BMP4 cKO mice compared to BMP4 control mice in this example with multiple measurements (P<0.0001 for the difference in CTR and BMP4 cKO) along the entire odontoblast area and confirmed visually in independent sets of control (CTR) and BMP4 cKO tissues at similar ages (data not shown) (E). d - dentin; od - odontoblasts (red arrows); ameloblasts (yellow arrows); p - dental pulp; ob - osteoblasts (black arrows); oc - osteocytes (blue arrows); b - bone; pd - periodontium. The dashed line (G) represents the boundary between the bone and periodontium in BMP4 cKO mice, which is clearly defined with blue stained osteoblasts in CTR mice.