Table 1. Primers used in this study.

The translation initiation codon sequence is shown in bold. Enzyme recognition sites are underlined. Mutated nucleotide is indicated in white character on black background

Name	Polarity	Sequence (5′→3′)
TV1.EcoRI.Kz.S	Sense	TATAGAATTCGCCACCATGCGCGTGATGGGCACCCAG
TV1.DraIII.AS	Antisense	TTCTCGGTCACGTTGCCCAGCA
5-wt L01	Sense	AATTCGCCACCATGCGCGTGAAGGGC
5-wt L02	Sense	ATCCGCAAGAACTACCAGCACCTGTGGCGCGGCGGCACCC
5-wt L03	Sense	TGCTGCTCCCGATGCTGATGATCTGCAGCGCCGTGGAGAA
5-wt L04	Sense	GCTGTGGGTGACCGTGTACTACGGCGTGCCCGTGTGGAAG
5-wt L05	Sense	GAGGCCACCACCACCCTGTTCTGCGCCAGCGACGCCAAG
5-wt L06	Sense	GCCTACGACACCGAGGTGCACAAC
3-wt L01	Antisense	GTGCACCTCGGTGTCGTAGGCCTTGGCGTCGCTGGCGCAGA
3-wt L02	Antisense	ACAGGGTGGTGGTGGCCTCCTTCCACACGGGCACGCCGT
3-wt L03	Antisense	AGTACACGGTCACCCACAGCTTCTCCACGGCGCTGCAGATC
3-wt L04	Antisense	ATCAGCATGCCCAGCAGCAGGGTGCCGCCGCGCCACAGGTG
3-wt L05	Antisense	CTGGTAGTTCTTGCGGATGCCCTTCACGCGCATGGTGGCG
SF162.BamHI.KO.S	Sense	AACCTGCTGCAGTACTGGATTCAGGAGCTGAAG
SF162.BamHI.KO.AS	Antisense	ATCCAGTACTGCAGCAGGTTGCCCCAGTAC
BGHpA.XhoI.S	Sense	TAACTCGAGCTGTGCCTTCTAGTTGCCAGC
BGHpA.XbaI.AS	Antisense	TAATCTAGACCATAGAGCCCACCGCATCCC
Ad5E3-P1	Sense	TACGAGAGAACCTCTCCGAG
Ad5E3-P2	Antisense	ACAGGCTGGCTCCTTAAAAT