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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2010 Apr 24;49(1):109–116. doi: 10.1016/j.freeradbiomed.2010.04.006

Figure 5.

Figure 5

DHODH RNA interference diminishes DHODH protein expression, and suppresses 4HPR-induced ROS and apoptosis in COLO 16 cells. (A) An assessment of 4HPR-induced ROS production as described in Fig. 3C following a 48-h treatments with the siRNA control or the siDHODH. *P<0.001 compared to the siRNA control and **P<0.001 compared to the siRNA control with 5 μM 4HPR. The inset figure is an immunoblot analysis of DHODH expression in the COLO 16 cells following the indicated 48-h treatments. (B) Representative epifluorescence images illustrate the nuclear morphology of the COLO 16 cells treated as described in (A) for 24 h without or with 5 μM 4HPR included. The scale bars equal 18 μm. (C) A summary of the condensed/fragmented nuclei for the COLO 16 cells in the treatment populations described in (B). *P<0.001 compared to the siRNA control and **P<0.01 compared to the siRNA control with 5 μM 4HPR.