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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Prostate. 2010 Jun 15;70(9):959–970. doi: 10.1002/pros.21130

Figure 3. Effects of androgens on the cellular distribution of SRC1, SRC2, SRC3, CBP and p300.

Figure 3

LNCaP cells were seeded in medium supplemented with CSS. Two days later, medium was changed and cells were treated with 1nM of the synthetic androgen R1881 or ethanol vehicle for 96 hours. Nuclear (nucl) and cytoplasmic (cyto) extracts were prepared and equal amounts of protein were analyzed by western blotting using antibodies directed against SRC1, SRC2, SRC3, CBP and p300. As controls for the efficacy of cell fractionation and androgen stimulation, expression of AR and beta-tubulin (β-tub) was also evaluated. To assess potential inter-sample loading differences, blots were stripped and reprobed with an antibody recognizing beta-actin (β-act).