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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Protein Expr Purif. 2010 Mar 18;73(1):78–82. doi: 10.1016/j.pep.2010.03.010

Fig 1.

Fig 1

UV-Vis spectra of gsNOS expressed alone (thin line) and gsNOS expressed with FC (thick line). Co-expression of FC results in a substantial increase in heme content of gsNOS as measured by the Abs403/Abs280 ratio. This value saturates at 0.6, which indicates nearly complete heme incorporation. Inset: GsNOS expressed by itself results in two bands on SDS-PAGE (lane A), both of which shift on His-tag cleavage (lane B). GsNOS co-expressed with FC results in just one band (lane C), that shifts on His-tag cleavage (lane D) as expected.