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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Protein Expr Purif. 2010 Mar 18;73(1):78–82. doi: 10.1016/j.pep.2010.03.010

Fig 3.

Fig 3

UV-Vis spectra of BP450 expressed by itself (thin line) and BP450 expressed with FC (thick line). Co-expression of FC results in a substantial increase in heme content of BP450 (AbsSoret/Abs280). Inset: BP450 expressed alone also results in two bands (lane A), both of which shift on His-tag cleavage (lane B). BP450 co-expressed with FC results in one band (lane C), which shifts on His-tag cleavage as expected (lane D).