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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Neurotoxicol Teratol. 2010 Mar 4;32(4):481–488. doi: 10.1016/j.ntt.2010.02.003

Figure 5. E10 neurospheres differentiate into neurons, astrocytes and oligodendrocytes.

Figure 5

E10 neurospheres were grown for 5 days with EGF and FGF2, then attached to Petri dishes and allowed to differentiate for 5 days without growth factors. Cells differentiated into (A,B) neurons (β III tubulin, green), (C) astrocytes (GFAP, red) and (D) oligodendrocytes (O4, red). Nuclei were stained using DAPI (blue). (E,F) Triple labeling of β III tubulin, GFAP and DAPI. (G) Triple labeling of β III tubulin, O4 and DAPI. GFAP, glial fibrillary acidic protein; DAPI, 4',6-diamidino-2-phenylindole.