CM-Dil labeled single cells were transplanted intra-cerebrally into 24h-old chicks and the cells were tracked 24h (A–C), 6 days (D,E) and 14 days (F) post transplantation. (A) Transplanted cells migrated and seen along the left cerebral cortex 24h post transplantation. (B) Transplanted cells migrated and seen around the left ventricle 24h post transplantation. (C) Transplanted cells migrated and seen inside the left ventricle 24h post transplantation. (D,E) Transplanted cells are seen around the left ventricle 6 days post transplantation. (F) Transplanted cells are seen along the left cerebral cortex 14 days post transplantation. (G) Quantification of in vivo cell survival 24h, 6 days and 14 days post transplantation. Transplanted cells – red (CM-Dil). Nuclei – blue (DAPI). h, hours Every bar represents two brains (total of 6). Error bars indicate SEM.