Fig. 2.

The level of the olfactory bulb GAD₆₇ is lower in the MAOB KO mouse than WT and is decreased in either animal after the Amph treatment. The mice received i.p. injections of saline (S) or Amph (A) for 3 days, and after the final injection on day 4, were subjected to brain dissection for removal of OlfBs. Gel electrophoresis and immunoblotting were conducted on the homogenates of the OlfBs by using an anti-GAD₆₇ antibody (a), as described in the Methods. Levels of GAD₆₇ are presented as percentages of that of the saline-treated WT (b). *: significantly different from the value of saline-treated WT mice, p<0.05; ⊚: significantly different from Amph-treated WT; #: significantly different from saline-treated MAOB KO, p<0.05.