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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Mar 17;1804(7):1443–1448. doi: 10.1016/j.bbapap.2010.03.004

Figure 2.

Figure 2

The 2.7 Å crystal structure of a tetrameric porcine group-IB sPLA2 complexed with 12 C8S molecules. A The C8S alkyl sulfates are clustered in the middle of the tetrameric quaternary structure, shown as white balls and sticks. The residues K10, R6 and the active site calcium of subunit A are shown in blue, while the active site calcium of subunits B, C and D are rendered white. This figure was made using the program MOLSCRIPT. B Molecular model of the alkyl sulfate and sPLA2 tetramer complex. The protein subunits A(green), B(cyan), C(yellow) and D(purple) are shown as a Ca trace. The interface residues shown in surface representations are A: L19, N24, L31, G32, V65, D66, N67 and T70; B: L2, H17, L19, M20, D21, F22, N23, N24, L31, D66, N67, T70, K113, K116, N117, and K121; C: W3, R6, K10, L19, M20, S60, K62, F63, D66, S74, N89, E92 and A93; D: W3, R6, K10, L19, M20, N23, N24, L31, K62, F63 and V65. The c8s molecules are shown in sphere/mesh surface representation. Also shown are Ca2+ ions (burgundy sphere), one Cl− ion (green sphere) and water molecules (blue sphere) that are within a 4 Å radius from the interface residues. This figure was made using the program PYMOL.