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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Prostate. 2010 Jun 15;70(9):1002–1011. doi: 10.1002/pros.21134

Figure 3. Castration of male PSA/A2.1 mice augments PSA-specific cellular immune responses.

Figure 3

Castrated male mice were immunized with either vac-mTag or vac-PSA and antigen-specific CD8 responses were evaluated by ELISPOT and tetramer staining. Non-castrated male mice and female mice were used as controls. For splenocyte restimulation in vitro, bone marrow-derived DC were loaded with either H-2Kb restricted-, SV40 Tag-derived epitope IV (Tag-IV/DC) (A), with PSA (PSA/DC) (B) or with HLA-A2.1-restricted, PSA-derived epitopes (PSA3A/DC) (C) and (PSA3/DC) (D). IFN-γ-releasing CD8 T cells were revealed using an ELISPOT assay. Data shown as Mean ± SD, representative of one experiment with from 3 animals/group.