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. Author manuscript; available in PMC: 2011 Jul 29.
Published in final edited form as: Mol Cell Endocrinol. 2010 Mar 17;323(2):268–276. doi: 10.1016/j.mce.2010.03.013

Fig 3. Overexpression of ERα46 inhibits E2-induced endogenous Cyclin D1 gene transcription in MCF-7 cells.

Fig 3

MCF-7 (ATCC), LCC9, and LY2 cells were transiently transfected with pCDNA3 parental vector or pCR3.1-hERα46 (Penot et al. 2005) for 30 h as described in Material and Methods. Cells were treated with EtOH, 10 nM E2, or 100 nM 4-OHT for 4 h. Q-RT-PCR analysis of cyclin D1 (CCND1) expression was normalized to 18S and the fold comparison was against EtOH for pcDNA 3.1-transfected cells within each cell line as described in Material and Methods. Values are the average ± SEM of three separate experiments. * Significantly different from EtOH control, p < 0.05. # Significantly different from pcDNA3-transfected control, p < 0.05.

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