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. Author manuscript; available in PMC: 2011 Jul 29.
Published in final edited form as: Mol Cell Endocrinol. 2010 Mar 17;323(2):268–276. doi: 10.1016/j.mce.2010.03.013

Fig. 4. Overexpression of ERα46 alters basal and E2-induced endogenous gene transcription in a gene- and cell- specific manner.

Fig. 4

The cell lines indicated (MCF-7 (ATCC) in A; and MCF-7 (ATCC), LCC9, and LY2 in B and C) were transfected with pcDNA3.1 (vector) or pcDNA3.1-ERα46 as described in Materials and Methods. Thirty h after transfection, cells were treated with EtOH, 10 nM E2, or 100 nM 4-OHT for 4 h. Q-PCR was performed to measure pS2/TTF1 and PGR (A), ESR2 (ERβ) (B), and NRF1 (NRF-1) (C) mRNA expression. The fold comparison was against EtOH for pcDNA 3.1-transfected cells within each cell line as described in Material and Methods. Values are the average +/− SEM of three separate transfection experiments in which all determinations were performed in triplicate. * Significantly different from EtOH control, p < 0.05, # Significantly different from the same treatment in pcDNA3.1-transfected cells.

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