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. Author manuscript; available in PMC: 2011 Jul 29.
Published in final edited form as: Mol Cell Endocrinol. 2010 Mar 17;323(2):268–276. doi: 10.1016/j.mce.2010.03.013

Fig. 6. Overexpression of ERα46 stimulates E2-induced endogenous miR-21 gene transcription and reduces miR-21 targets PTEN and PDCD4 in MCF-7 cells.

Fig. 6

MCF-7 (ATCC), LCC9, and LY2 cell lines were transfected with pcDNA3.1 (vector) or pcDNA3.1-ERα46 as described in Materials and Methods. Thirty h after transfection, cells were treated with EtOH, 10 nM E2, or 100 nM 4-OHT for 4 h. Q-PCR was performed to measure miR-21 normalized to 5S RNA (A). Q-PCR was performed to measure PTEN and PDCD4 relative to 18S in MCF-7 cells transfected and treated as indicated. Values are the average +/− SEM of three separate transfection experiments in which all determinations were performed in triplicate. * Significantly different from EtOH control, p < 0.05. # Significantly different from the same treatment in pcDNA3.1-transfected cells, p < 0.05.

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