MCF-7 (ATCC) cells were transfected with pcDNA3.1 or pcDNA3.1-ERα46. Twenty-four h after transfection, cells were treated with EtOH or 10 nM E2 for 24 h. 5 μg WCE were slot blotted onto PVDF membranes, stained with Ponceau S, washed and probed for Pten (A) or Pdcd4 (B). Values are the average +/− Std dev of duplicate samples. * Significantly different from EtOH control, p < 0.05, # Significantly different from the same treatment in pcDNA3.1-transfected cells.