Figure 2. Effect of EGFP siRNA loaded nanoparticles on BHK-21-EGFP cells was assessed.

EGFP siRNA was efficiently incorporated into cells and effectively inhibited the expression of EGFP in a dose dependent manner (NP: nanoparticle). siPORT™ NeoFX™ Transfection Agent (Ambion) was used as a positive control. Nanoparticles loaded with 100 nM non-specific siRNA (c-siRNA) and unloaded nanoparticles were used as negative controls.