Figure 4. Western blot assay was performed to assess the duration of MDR1 siRNA loaded nanoparticle.

siRNA transfected with siPORT™ NeoFX™ Transfection Agent was able to suppress P-gp expression for 48 hours (A). Inhibition of P-gp started slower when MDR1 siRNA was loaded in to nanoparticles, but exhibited longer inhibition compared to MDR1 siRNA transfected with commercially available agent (B). P values are shown as follows: *P<0.05.