Figure 7. The effect of doxorubicin alone or nanoparticle loaded with MDR1 siRNA on KHOS_R2 and U-2OS_R2 was analyzed.

Varying concentrations of doxorubicin was added and cells were cultured for 5 days. Treatment with nanoparticles loaded with MDR1 siRNA showed increased anti-proliferative activity in both drug resistant osteosarcoma cell lines, KHOS_R2 (A) and U-2OS_R2 (B), in a dose dependent manner. Growth inhibition was assessed by MTT as described under Materials and Methods. The experiment was repeated four times in triplicate.