Figure 3. Injection of B7-H1^−/− DC results in reduced frequency of neurantigen-specific IFN-γ and IL-17 secreting cells in the CNS.

MOG_35–55 loaded and TNF-matured WT or B7-H1^−/− DC (2,5×10⁶) were injected i.v. into WT mice 7, 5 and 3 days before EAE induction. Control mice were injected with PBS. After 15 days CNS mononuclear cells were harvested and CNS T cell infitration and cytokine production were investigated using flow cytometry and ELISPOT assay. A) Dot blots are gated for live lymphocytes and inserted numbers represent the percentage of CD4⁺ and CD8⁺ CNS infiltrating T cells of 3–5 pooled mice per group of one experiment. Results are representative for 2 independent experiments. B) ELISPOT analysis of IFN-γ (left) or IL-17 (right) production by infiltrating CNS cells after MOG_35–55 peptide restimulation. Results are representative for 2 independent experiments with 3–5 pooled mice per group. * p<0.05, ** p<0.01, n.s. not significant.