Table 1. Bacterial strains, plasmids and primers used in this work.
Strain/plasmid | Relevant genotype and description | Source/reference |
Strains | ||
Xanthomonas axonopodis pv. citri | ||
Xcc99-1330 | Wild type, Apr | B. I. Canteros |
XackatE | katE mutant of Xcc99-1330, Kmr, Apr | This work |
cXackatE | XackatE complemented, carries pBBR1/katE, Kmr, Gmr, Apr | This work |
Escherichia coli | ||
JM109 | HsdR17 endA1 Recal thi gyrA96 relA1 recA1 supE44 λ−Δ(lac-proAB), [F′, traD36, proA+B+, lacIqZΔM15] | [16] |
S17-1 | thi, pro, hsdR, recA with RP4-2[Tc::Mu-Km::Tn7], Smr | [21] |
Plasmids | ||
pGEM-T Easy | PCR cloning and sequencing vector, Apr | Promega |
pGEM/katE | pGEM-T Easy containing 440-bp fragment of katE | This work |
pK18mobGII | pUC18 derivative, lacZa, gusA, mob site, Kmr | [24] |
pKmob/katE | pK18mobGII containing 440-bp fragment of katE | This work |
pBBR1MCS-5 | Broad host-range vector, Gmr | [25] |
pBBR1/katE | pBBR1MCS-5 containing katE gene | This work |
Ap, ampicillin; Km, kanamycin; Gm, gentamycin; Sm, streptomycin.
a. Capital letters correspond to nucleotides of the Xac genome sequence and small letters to nucleotides added to facilitate cloning.