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. 2010 May 24;5(5):e10803. doi: 10.1371/journal.pone.0010803

Table 1. Bacterial strains, plasmids and primers used in this work.

Strain/plasmid Relevant genotype and description Source/reference
Strains
Xanthomonas axonopodis pv. citri
Xcc99-1330 Wild type, Apr B. I. Canteros
XackatE katE mutant of Xcc99-1330, Kmr, Apr This work
cXackatE XackatE complemented, carries pBBR1/katE, Kmr, Gmr, Apr This work
Escherichia coli
JM109 HsdR17 endA1 Recal thi gyrA96 relA1 recA1 supE44 λΔ(lac-proAB), [F′, traD36, proA+B+, lacIqZΔM15] [16]
S17-1 thi, pro, hsdR, recA with RP4-2[Tc::Mu-Km::Tn7], Smr [21]
Plasmids
pGEM-T Easy PCR cloning and sequencing vector, Apr Promega
pGEM/katE pGEM-T Easy containing 440-bp fragment of katE This work
pK18mobGII pUC18 derivative, lacZa, gusA, mob site, Kmr [24]
pKmob/katE pK18mobGII containing 440-bp fragment of katE This work
pBBR1MCS-5 Broad host-range vector, Gmr [25]
pBBR1/katE pBBR1MCS-5 containing katE gene This work

Ap, ampicillin; Km, kanamycin; Gm, gentamycin; Sm, streptomycin.

a. Capital letters correspond to nucleotides of the Xac genome sequence and small letters to nucleotides added to facilitate cloning.