Table 1.

In vitro dynamic test of cytokine adsorption by Amberchrom CG161 M resin

Cytokine	Concentration (pg/ml)	Amount bound (% leakage) (pg/ml adsorbent)	Theoretical binding capacity (pg/ml adsorbent)
TNF-α	675 ± 72	4999 (5%) ± 1703	200,980 ± 53,607
IL-1-β	331 ± 1	61,439 (5%) ± 10,459	116,842 ± 24,707
IL-6	334 ± 59	35,729 (5%) ± 8149	912,776 ± 200,096
IL-8	340 ± 186	322 (12%) ± 161	48,940 ± 26,089
IL-10	464 ± 143	69,187 (5%) ± 31,112	972,880 ± 61,006

The conditions used for the dynamic studies on Amberchrom CG161 M resin are extensively described in the Materials and Methods. Briefly, the resin columns were perfused at a low linear velocity (0.30 ml/min). The theoretical binding capacity of the resins for individual cytokines which was calculated by extrapolation of the increase in cytokine concentration at the column outlet over time only gives a rough estimation of the actual maximal binding capacity.