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Multiphoton microscopy images obtained from (a) SKG mouse vertebrae and (b) BALB/c mouse vertebrae. The green color corresponds to backward SHG channel and the blue color to forward SHG channel. (c) The collagen organization, represented by the forward to backward ratio, was changed by arthritis. Scale bars = 100 μm. *P < 0.05. SHG, second-harmonic generation.
