Table 1.
Ovine-specific real time PCR primer pair sequences, annealing temperatures and product size
| Target gene | Sequence | Anneal temp (°C) | Product size (bp) | Accession number or reference if published |
|---|---|---|---|---|
| Acan | F - TCA CCA TCC CCT GCT ACT TCA TC R - TCT CCT TGG AAA TGC GGC TC |
58 | 105 | [21] |
| Adamts1 | F - CCA ACT GGA GCC ACA AAC ATT G R - GGA CAG AGT GAA GTC GCC ATT C |
55 | 126 | [GenBank: XM_589626] |
| Adamts4 | F - AAC TCG AAG CAA TGC ACT GGT R - TGC CCG AAG CCA TTG TCT A |
60 | 149 | [44] |
| Adamts5 | F - GCA TTG ACG CAT CCA AAC CC R - CGT GGT AGG TCC AGC AAA CAG TTA C |
55 | 97 | [21] |
| Col2a1 | F - TGA CCT GAC GCC CAT TCA TC R - TTT CCT GTC TCT GCC TTG ACC C |
55 | 154 | [GenBank: X02420] |
| Mmp1 | F - CAT TCT ACT GAC ATT GGG GCT CTG R - TGA GTG GGA TTT TGG GAA GGT C |
55 | 122 | [GenBank: AF267156] |
| Mmp3 | F - TCC CCC AGT TTC CCC TAA TG R - GAT TTC TCC CCT CAG TGT GCT G |
58 | 124 | [GenBank: AF135232] |
| Mmp13 | F - GGT GAC AGG CAG ACT TGA TGA TAA C R - ATT TGG TCC AGG AGG GAA AGC G |
58 | 349 | [21] |
| Mmp14 | R - CCC AGT GCT TGT CTC CTT TGA AG | 56 | 126 | [GenBank: AF267160] |
| Timp1 | F - GGT TCA GTG CCT TGA GAG ATG C R - GGG ATA GAT GAG CAG GGA AAC AC |
57 | 265 | [GenBank: S67450] |
| Timp2 | F - ACT CTG GCA ACG ACA TCT ACG G R - TCT TCT TCT GGG TGG CAC TCA G |
57 | 261 | [GenBank: M32303] |
| Timp3 | F - CTT CCT TTG CCC TTC TCT ACC C R - TCT GGT CAA CCC AAG CAT CG |
57 | 286 | [GenBank: NM_174473] |
| Gapdh | F - CCT GGA GAA ACC TGC CAA GTA TG R - GGT AGA AGA GTG AGT GTC GCT GTT G |
58 | 139 | [GenBank: U94889] |
Acan = aggrecan; Adamts = a disintegrin and metalloproteinase with thrombospondin motifs; F = forward primer; Gapdh = glyceraldehyde 3-phosphate dehydrogenase; Mmp = matrix metalloproteinase; R = reverse primer; Timp = tissue inhibitor of metalloproteinases.