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CCL2 is stored in cytoplasmic vesicles in fibroblast-like synoviocytes (FLS). FLS were cultured for 24 hours in the absence (a, c) or presence (b) of 50 μg/ml monosodium urate (MSU) crystals. Cells were subjected to immunostaining with anti-CCL2 antibodies (a, b) or incubated with the second antibody only as a negative control (c), and then analyzed with confocal microscopy, as described in Materials and Methods. Original magnification, ×1,000.
