Table 1.
Primer sequences used for real-time PCR to evaluate gene expression by mesenchymal precursor cells
| Gene | Primer sequences (5' to 3') |
|---|---|
| Aggrecan | Forward: CTG CTT CCG AGG CAT TTC |
| Reverse: GCT CGG TGG TGA ACT CTA GG | |
| Type I collagen | Forward: AGG GTC CCA ACG AGA TCG AGA TCC |
| Reverse: TAC AGG AAG CAG ACA GGG CCA ACG TCG | |
| Type II collagen | Forward: GCC TGG TGT CAT GGG TTT |
| Reverse: GTC CCT TCT CAC CAG CTT TG | |
| Sox-9 | Forward: AGG TGC TCA AAG GCT ACG AC |
| Reverse: GCT TCT CGC TCT CGT TCA GA | |
| Type X collagen | Forward: AAT GCC CAC AGG CAT AAA AG |
| Reverse: AGG ACT TCC GTA GCC TGG TT | |
| IGFBP-3 | Forward: CGC CAG GAA ATG CTA GTG AG |
| Reverse: ACG GCA GGG ACC ATA TTC T | |
| IGFBP-5 | Forward: GAG AAA GCC CTC TCC ATG TG |
| Reverse: TCA CGG GAG TCT CTC TCG AT | |
| Noggin | Forward: ATC GAA CAC CCA GAC CCT ATC |
| Reverse: TCT AGC CCT TTG ATC TCG CTC | |
| CBFA-1 (RUNX2) | Forward: GTG GAC GAG GCA AGA GTT TCA |
| Reverse: CAT CAA GCT TCT GTC TGT GCC | |
| β-Actin | Forward: GAT CAT TGC TCC TCC TGA GC |
| Reverse: GTC ATA GTC CGC CTA GAA GCA T |
Primer sequences used for real-time PCR to evaluate gene expression by mesenchymal precursor cells maintained in micromass cultures in the absence and presence of pentosan polysulfate for 7 and 10 days. IGFBP, insulin-like growth factor binding protein.