Table 2.
PPS concentration (μg/ml) | HA concentration (μg/ml) | |||
---|---|---|---|---|
Media 7 days | Matrix 7 days | Media 10 days | Matrix 10 days | |
Control 0.0 | 1.54 ± 0.03 | 1.58 ± 0.01 | 1.55 ± 0.06 | 2.10 ± 0.03§ |
0.1 | 1.34 ± 0.05# | 1.76 ± 0.26 | 1.30 ± 0.08# | 1.64 ± 0.07# |
0.5 | 1.34 ± 0.11# | 1.74 ± 0.07 | 1.22 ± 0.17# | 1.89 ± 0.01# |
1.0 | 1.09 ± 0.05# | 1.98 ± 0.29 | 1.23 ± 0.06# | 2.42 ± 0.32 |
2.5 | 1.50 ± 0.17 | 1.78 ± 0.23 | 1.32 ± 0.12# | 2.24 ± 0.39 |
5.0 | 1.28 ± 0.05# | 2.16 ± 0.20* | 1.33 ± 0.07# | 2.27 ± 0.24 |
10.0 | 1.25 ± 0.03# | 2.39 ± 0.32 | 0.84 ± 0.09# | 2.57 ± 0.31 |
20.0 | 1.36 ± 0.05# | 2.31 ± 0.33* | 1.32 ± 0.06# | 2.54 ± 0.04** |
Statistical significance | #PPS < control (P < 0.05) | *PPS > control (P < 0.05) | #PPS < control (P < 0.05) | §Matrix > media (P < 0.05), #PPS < control (P < 0.05), **PPS > control (P < 0.005) |
Concentration effects of pentosan polysulfate (PPS) on hyaluronan (HA) deposition in the media and matrix of 7-day and 10-day micromass cultures of human mesenchymal progenitor cells (MPCs). Data are mean ± standard deviation of triplicate culture values.