A. Neurons cultured in microfluidic device at DIV 8–11 stained for β-Tubulin. B. Neurons stained for MAP-2. C, D. Neurons stained for Tau-1 in microfluidic device (C) or in the non-seeded chamber (D). E, F. Neurons stained for NHE-1 protein in the seeded- (E) and non-seeded chambers (F). Inset, negative controls by omitting primary antibodies for β-Tubluin, MAP-2, Tau-1 or NHE-1 antibody. Scale bars = 100 µm in A, B, C. and = 50 µm in D, E, F.