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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Eur J Neurosci. 2009 Sep 24;30(7):1358–1369. doi: 10.1111/j.1460-9568.2009.06904.x

Figure 2. Microinjection of the AMPA/kainate receptor antagonist CNQX into the PPTg/LDT dose-dependently attenuated reinstatement of drug seeking induced by a priming injection of cocaine.

Figure 2

(A) Data in panel A depict the total responses (mean±S.E.M.) on the active and inactive levers following a systemic priming injection of cocaine (10 mg/kg, i.p.) in animals pretreated with microinfusions of saline (n = 9), 0.03 (n = 6) or 0.3 μg (n = 9) CNQX into the PPTg/LDT. The asterisk represents a significant difference between responses on the active lever following 0.3 μg CNQX and saline or 0.03 μg CNQX pretreatments (Tukey’s HSD, P<0.05). No significant differences in responding on the inactive lever (mean±S.E.M.) were found during the reinstatement phase of the experiment when saline, 0.03 or 0.3 μg CNQX were administered into the PPTg/LDT 10 minutes prior to a systemic injection of cocaine (10 mg/kg, i.p.). (B) The time courses of active lever responding (mean±S.E.M.) following intra-PPTg/LDT administration of saline (n = 9) or 0.3 μg CNQX (n = 9) prior to a systemic priming injection of cocaine (10 mg/kg i.p.). (C) Intra-PPTg/LDT administration of CNQX did not affect food-seeking behavior in rats. Animals microinjected with saline (n = 6) or 0.3 μg CNQX (n = 6) directly into the PPTg/LDT prior to the reinstatement of food seeking displayed no difference in total active lever responses between treatments. (D) Coronal sections depicting microinjection sites, as indicated by the closed circles, targeting the PPTg/LDT. Numbers on the left side of the coronal sections denote distance from bregma in the anteroposterior direction. The insert displays a magnified (4x) microinfusion site within the PPTg (the ventral tip of the microinjector is indicated by the arrow).