Fig. 6.

Effects of different treatments on four parameters measured simultaneously in RLM. Traces ‘a’, MPT induction by Ca²⁺; traces ‘b’, effect of minocycline; traces ‘c’, inhibitory effect of Ru360 on MPT. Experimental conditions: sucrose buffer contained 300 mM sucrose, 3 mM Hepes, 2.5 mM KH₂PO₄, and 5 mM succinate plus 1 µM rotenone, pH 7.4. The RLM concentration was 0.45 mg/ml. Additions of Ca²⁺, minocycline (M) and Ru360 (Ru) were 60, 100 µM and 100 µg/ml, respectively. Panels A–D: O₂ uptake, Ca²⁺ flux, ΔΨ, and swelling, respectively. (Panel A) The decrease in O₂ concentration reflects O₂ uptake by mitochondria. (Panel B) The increase in Ca²⁺ concentration indicates either Ca²⁺ efflux from mitochondria or absence of Ca²⁺ influx into mitochondria. (Panel C) The increase in TPP⁺ concentration reflects ΔΨ dissipation. (Panel D) The decrease in absorbance indicates mitochondrial swelling. The traces are representative of at least four experiments obtained with different mitochondrial preparations