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. 2010 Apr 14;24(6):1187–1202. doi: 10.1210/me.2009-0480

Figure 2.

Figure 2

Interaction of ERα, SRC-3, and SMRT with enhancer regions of the cyclin D1 and PR genes. Chromatin was prepared from MCF-7 cells treated with vehicle (Veh), 10 nm E2, or 100 nm 4HT for 45 min and then subjected to ChIP assay using antibodies for ERα (top), SMRT (middle), or SRC-3 (bottom) in parallel to the appropriate IgG negative control. Immunoprecipitated DNA was quantitated by qPCR using primers to amplify enhancer-2 of the cyclin D1 gene (A) or the ER_7204 binding site in the PR gene (B). Data represent an average ± sem of three independent experiments.