Figure 6.

SMRT binds directly to SRC-3 in vitro. Panel A, Schematic diagram showing SMRT_short and the GST-tagged fragments of SMRT (A–D) used in the experiment (left). GST pull-down assay demonstrating the interaction of full-length in vitro-transcribed and -translated SRC-3 with GST-SMRT fusion proteins or GST control (right). Panel B, Schematic representation of full-length SRC-3 and the GST-tagged SRC-3 fragments used in this study (left). Interaction of in vitro-transcribed and -translated SMRT_short with GST-SRC-3 fusion proteins or GST control (right). Panel C, Interaction of in vitro-translated S/T or HAT domains of SRC-3 with GST-SMRT fusion proteins or GST control. In each assay, bound ³⁵S-labeled proteins were resolved by SDS-PAGE and detected by autoradiography. Input lanes represent 10% of ³⁵S-labeled full-length SRC-3 or fragments (S/T or HAT) of SRC-3 or 0.5% of SMRT_short proteins used in pull-down assays. Results are representative of three independent experiments.