Figure 10.

c-Myc is required for the glucose-mediated recruitment of ChREBP to ChoREs. INS-1-derived 832/13 cells were treated for 16 h with a vehicle control (DMSO) or the indicated concentrations of 1RH in either 2 or 20 mm glucose as in Fig. 2. Total RNA was isolated and subjected to RT-PCR using primers specific for Slc2a4 (A), glycerol 3 phosphate dehydrogenase (GPDH) (B), Prok2 (C), and β-actin. Results are the mean ± se (n = 3). D–G, Cells were pretreated for 16 h with 40 μm 1RH or vehicle control and then treated for 6 h with either 2 or 20 mm glucose as in Fig. 2. A ChIP assay was performed with antibodies directed against ChREBP or an IgG control. PCR was performed using primers specific for the regions flanking the ChoREs of the indicated genes. The results are expressed as the mean of three independent experiments ± se. #, P < 0.05 for 2 mm glucose vs. 20 mm glucose; *, P < 0.05 for 20 mm glucose vehicle control vs. 20 mm glucose and 1RH.