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. 2010 Apr 9;24(6):1297–1304. doi: 10.1210/me.2010-0068

Figure 2.

Figure 2

Expression of transgenic human COUP-TFI after cre-mediated activation. Animals that carried both the R26creERT2 and CAG-S-hCOUP-TFI alleles were given oil and tamoxifen for control and activation, respectively. A, Western blot of E15.5 embryonic extracts with and without activation at E12.5. The membrane was blotted with anti-COUP-TFI, anti-myc, and anti-FLAG antibodies to detect transgenic (Tg) or endogenous (Wt) COUP-TFI expression. B, Immunofluorescence staining with anti-COUP-TFI and anti-myc antibodies on tissue sections of E12.5 pharyngeal arches with and without activation at E9.5. C, Immunofluorescence staining with the anti-myc antibody on E12.5 tissue sections of various organs after activation at E9.5.