Figure 3.

CHD8 activates AR-mediated transcription of the TMPRSS2 gene. LNCaP cells were transfected with the indicated siRNA constructs. After selection of the transfected cells, cultures were treated with ethanol (EtOH) or 10 nm DHT for 6 h. A and B, Total RNA was isolated, and TMPRSS2 expression (A) or PSA expression (B) was analyzed by quantitative RT-PCR. *, P < 0.05; **, P < 0.01 by Student's t test. C, Efficiency of CHD8 knockdown was determined by Western blot analysis with CHD8 antibodies. Actin is blotted as a loading control.