Figure 5.

CHD8 is required for optimal androgen-responsive binding of AR to the TMPRSS2 ARE. LNCaP cells were transfected with the indicated siRNA constructs. After selection of the transfected cells, cultures were treated with ethanol or 10 nm DHT for 6 h. Chromatin was cross-linked in vivo with formaldehyde. Cells were lysed, and ChIP was performed with the indicated antibodies. Bound DNA was detected by quantitative PCR using primers to the TMPRSS2 ARE. Control IgG-precipitated samples were less than 0.005% of input and therefore are not shown. Shown is a typical result from multiple experiments.