Figure 6.

CHD8 regulates androgen-dependent cell proliferation. LNCaP cells were transfected with the indicated siRNA constructs. After selection of the transfected cells, cultures were treated with ethanol (EtOH) or 4 nm R1881. At the indicated time points, proliferation was determined using a luminescent-based assay of metabolically active cells. A, Data are expressed as relative proliferation, which is calculated as the luminescent signal for each condition normalized to the uninduced sample at d 1. B, Data are expressed as fold R1881-induced proliferation, which is calculated as the ratio of the luminescent signals from the induced to the uninduced cells for each siRNA treatment at each indicated time point. *, P < 0.02 by Student's t test.