SB431542, but not SIS, inhibited EGFR signaling in WT COCs. A, Egfr mRNA expression in COCs treated with DMSO, SB431542, or SIS for 20 h. B, Western blot analysis of EGFR, p-SMAD2, and β-actin (ACTB) in the same experimental groups as stated in panel A 20 h after culture. C, Changes of the levels of p-MAPK3/1 in the same experimental groups as stated in panel A after the initial 20-h culture and then treated with or without (control) AREG for 30 min. Groups indicated with different letters are significantly different, P < 0.05.