Figure 4.

Hepatic steatosis was accelerated by HFD in SIRT1^+/− mice. The liver was examined in the SIRT1^+/− mice on HFD for 16 wk (28 wk of age). A, Increased liver weight and size in the SIRT1^+/− Tg mice. B, TAG, glycerol, and cholesterol content in liver. C, H&E staining of liver. Pictures were taken under a microscopy with object lenses of ×10 or ×40, respectively. D, Gluconeogenic and lipogenic gene mRNA in liver. E, Inflammatory gene mRNA in liver. F, mRNA in liver. Values are the means ± se (n = 7). *, P ≤ 0.05; **, P ≤ 0.001.